Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune
Zhe Ji1, Chenghua Zhou2, Hongshen Niu3
1Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine; Department of Microbiology and Immunology, Tongji University School of Medicine; jizhe@sibs.ac.cn.
Abstract:
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) caused by the combination of environmental factors and susceptible genetic background. Experimental autoimmune encephalomyelitis (EAE) is a typical disease model of MS widely used for investigating the pathogenesis in which T lymphocytes specific for myelin antigens initiate an inflammatory reaction in CNS. It is very important to assess how lymphocytes in the CNS regulate the development of disease. However, the approach for measuring the quantity and quality of infiltrated lymphocytes in the CNS is very limited due to the difficulties in isolating and detecting infiltrated lymphocytes from the brain. This manuscript presents a protocol for that is useful for the isolation, identification, and characterization of infiltrated lymphocytes from the CNS and will be helpful for our understanding of how lymphocytes are involved in the development of the CNS autoimmune disease.
Insights
This study presents a new protocol to isolate and analyze T lymphocytes in the central nervous system (CNS). This method aids in understanding how these cells contribute to autoimmune diseases like multiple sclerosis (MS).
Area of Science:
- Neuroimmunology
- Autoimmune Diseases
- Central Nervous System (CNS) research
Background:
- Multiple sclerosis (MS) is a CNS autoimmune disease influenced by genetics and environment.
- Experimental autoimmune encephalomyelitis (EAE) models MS, involving myelin-specific T lymphocytes initiating CNS inflammation.
- Assessing CNS lymphocyte roles in disease development is crucial but challenging due to isolation difficulties.
Purpose of the Study:
- To present a novel protocol for isolating, identifying, and characterizing lymphocytes infiltrated in the CNS.
- To facilitate a deeper understanding of lymphocyte involvement in CNS autoimmune disease pathogenesis.
Main Methods:
- Development of a refined protocol for lymphocyte isolation from the CNS.
- Implementation of identification and characterization techniques for infiltrated lymphocytes.
Main Results:
- The presented protocol enables effective isolation of lymphocytes from the CNS.
- The method allows for detailed characterization of these infiltrated immune cells.
Conclusions:
- This protocol overcomes limitations in measuring CNS lymphocyte quantity and quality.
- It provides a valuable tool for studying the role of lymphocytes in CNS autoimmune diseases like MS and EAE.
More Related Videos
09:01Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
Published on: November 17, 2020
08:17Author Spotlight: Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis
Published on: February 23, 2024
