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Updated: Nov 27, 2025

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Published on: September 17, 2016
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock
Joel Heisler1,2,3, Archana Chavan3, Yong-Gang Chang1,3
1Chemistry and Chemical Biology, University of California, Merced, CA, USA.
Abstract:
Stochastic diffusion of a solution of fluorophores after photoselection reduces the polarization of emission, or fluorescence anisotropy. Because this randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.
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