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Related Experiment Video

Updated: Nov 27, 2025

Breath Collection from Children for Disease Biomarker Discovery

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

Circadian Metabolomics from Breath.

Steven A Brown¹, Pablo Sinues^2,3

¹Chronobiology and Sleep Research Group, Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)

|December 7, 2020

Summary

Circadian rhythms, or inner time, can be precisely determined using fluctuating biomolecules. New breath analysis techniques offer noninvasive insights into circadian metabolism.

Keywords:

Breath analysis Metabolomics Real-time analysis Secondary electrospray ionization

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Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

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Last Updated: Nov 27, 2025

Breath Collection from Children for Disease Biomarker Discovery

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Published on: April 7, 2020

Area of Science:

Biochemistry
Chronobiology
Metabolomics

Background:

Melatonin and other metabolites are crucial for determining circadian phase.
Comprehensive metabolome analyses reveal numerous small biomolecules with circadian fluctuations.
Understanding these fluctuations enhances our knowledge of the molecular basis of the circadian clock.

Purpose of the Study:

To explore the potential of analyzing exhaled metabolites for noninvasive, in vivo insights into circadian metabolism.
To highlight the role of advanced analytical techniques in circadian research.

Main Methods:

Utilizing comprehensive metabolome analyses.
Employing emerging analytical techniques for determining exhaled metabolites in breath.

Main Results:

Identification of panels of small biomolecules exhibiting circadian fluctuations.
Demonstration of the potential for noninvasive breath analysis in circadian studies.

Conclusions:

Circadian phase can be precisely determined by analyzing fluctuating biomolecules.
Exhaled metabolite analysis presents a promising noninvasive approach for in vivo studies of circadian metabolism.