H₂S oxidation coupled to nitrate reduction in a two-stage bioreactor: Targeting H₂S-rich biogas desulfurization

A two-stage bioreactor operated under anoxic denitrifying conditions was evaluated for desulfurization of synthetic biogas laden with H₂S concentrations between 2500 and 10,000 ppm_v. H₂S removal efficiencies higher than 95% were achieved for H₂S loads ranging from 16.2 to 51.9 gS m_liquid^-3h^-1. Average H₂S oxidation performance (fraction of S-SO₄^2- produced per gram of S-H₂S absorbed) ranged between 8.2 ± 1.2 and 18.7 ± 5.3% under continuous liquid operation. Nitrogen mass balance showed that only 2-6% of the N-NO₃^- consumed was directed to biomass growth and the rest was directed to denitrification. Significant changes in the bacterial community composition did not hinder the H₂S removal efficiency. The bioreactor configuration proposed avoided clogging issues due to elemental sulfur accumulation as commonly occurs in packed bed bioreactors devoted to H₂S-rich biogas desulfurization.