Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.3K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.1K
CRISPR01:59

CRISPR

55.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
55.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.2K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

432
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
432

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The phenotypic landscape of the model firmicute Bacillus subtilis.

bioRxiv : the preprint server for biology·2026
Same author

Predicting the protein interaction landscape of a free-living bacterium with pooled-AlphaFold3.

Molecular systems biology·2026
Same author

The phenotypic landscape of the mycobacterial cell.

bioRxiv : the preprint server for biology·2025
Same author

Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle.

Cell systems·2025
Same author

Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi.

Cell systems·2025
Same author

<i>Bacillus subtilis</i> in defense mode: Switch-like adaptations to protistan predation.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

A conceptual framework to dissect emergent functions in microbial communities.

Current opinion in microbiology·2026
Same journal

Differential signaling roles of the KinA-KinE sensor kinases in regulating Spo0A, the master regulator of Bacillus subtilis cell‑fate decisions.

Current opinion in microbiology·2026
Same journal

S-layers as natural building blocks for nanobiotechnology and synthetic biology.

Current opinion in microbiology·2026
Same journal

The role of the antimicrobial peptide nisin as a clean label food preservative.

Current opinion in microbiology·2026
Same journal

From coarse-grained metabolic rules to fine-grained control of microbial communities.

Current opinion in microbiology·2026
Same journal

Progress in engineered bacterial cancer therapies.

Current opinion in microbiology·2026
See all related articles

Related Experiment Video

Updated: Nov 27, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

811

Bacterial CRISPR screens for gene function.

Horia Todor1, Melanie R Silvis1, Hendrik Osadnik1

  • 1Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA.

Current Opinion in Microbiology
|December 7, 2020
PubMed
Summary
This summary is machine-generated.

CRISPR interference (CRISPRi) tools are advancing functional genomics screens in bacteria. Recent developments enable essential gene and genome-scale screens, even in challenging bacterial species.

More Related Videos

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.7K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.0K

Related Experiment Videos

Last Updated: Nov 27, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

811
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.7K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.0K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • CRISPR tools offer powerful methods for bacterial functional genomics.
  • CRISPR interference (CRISPRi) is a key technology for gene function studies.
  • Challenges remain in applying these tools to non-model and pathogenic bacteria.

Purpose of the Study:

  • To review the application of CRISPR tools, particularly CRISPRi, for functional genomics screens in bacteria.
  • To highlight recent advancements in CRISPRi technology and its screening capabilities.
  • To provide a forward-looking perspective on CRISPRi strategies for bacterial gene function determination.

Main Methods:

  • Review of recent literature on CRISPRi applications in bacterial functional genomics.
  • Discussion of CRISPRi titration techniques for essential gene screens.
  • Summary of genome-scale pooled CRISPRi screening approaches.
  • Overview of new dCas9 variants for enhanced CRISPRi functionality.

Main Results:

  • CRISPRi titration has enabled high-throughput essential gene functional screens.
  • Genome-scale pooled CRISPRi screens are becoming increasingly feasible.
  • Progress is being made in applying CRISPRi to non-model and pathogenic bacteria.
  • Development of novel dCas9 variants expands CRISPRi capabilities.

Conclusions:

  • CRISPRi is a versatile and powerful tool for bacterial functional genomics.
  • Continued development of CRISPRi technologies will accelerate gene function discovery in diverse bacteria.
  • Future strategies will focus on optimizing CRISPRi for broader applicability and deeper insights into bacterial biology.