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Updated: Nov 27, 2025
Diffusion Imaging in the Rat Cervical Spinal Cord
Published on: April 7, 2015
Evaluation of the effect of myelotomy on nerve function in rats with spinal cord injury by diffusion tensor imaging
Yi Liu1,2,3,4,5, Changbin Liu6, Chuan Qin1,2,3,4,5
1School of Rehabilitation Medicine, Capital Medical University, Beijing, PR China.
Background:
Spinal cord injury (SCI) is a severe central nervous system injury that can generally induce different degrees of sensory and motor dysfunction.
Purpose:
To clarify the changes of diffusion tensor imaging (DTI) parameters after spinal cord myelotomy in rats with SCI.
Material And Methods:
Eighteen Sprague Dawley (SD) rats were randomly divided into the Sham group (n=6), SCI group (n=6), and Mye group (n=6), respectively. The DTI values at 1, 3, 7, and 21 days after modeling were collected by magnetic resonance imaging (MRI). The spinal specimen at the injury site was collected on the 21st day for Nissl's staining to assess the changes in neurons.
Results:
The fractional anisotropy (FA) values in both the SCI group and Mye group significantly decreased. In addition, the FA values between the two groups were statistically significant (P < 0.001). The apparent diffusion coefficient (ADC), mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) values all decreased and then increased (P < 0.001). Pearson correlation test showed that the ADC, MD, and AD values were positively correlated with the Basso Beattie Bresnahan (BBB) score. Nissl's staining showed a higher number of Nissl's bodies, and deep staining of Nissl's bodies in the Mye group, while the morphology of neurons was relatively good. The number of neurons in the Mye group was significantly higher after myelotomy compared to the SCI group (P < 0.001).
Conclusion:
The DTI parameters, especially ADC values, could non-invasively and quantifiably evaluate the efficacy of myelotomy for rats with SCI.
