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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

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Related Experiment Video

Updated: Nov 26, 2025

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Does RNAi-Based Technology Fit within EU Sustainability Goals?

Clauvis Nji Tizi Taning¹, Bruno Mezzetti², Gijs Kleter³

¹Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Trends in Biotechnology

|December 8, 2020

Summary

This summary is machine-generated.

Research and development for pesticide alternatives is needed. RNA interference (RNAi) technology offers a promising solution for reducing pesticide risks and supporting sustainable agriculture.

Keywords:

Green Deal RNA interference agriculture integrated pest management pesticides sustainability

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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

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Related Experiment Videos

Last Updated: Nov 26, 2025

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

Area of Science:

Agricultural Science
Biotechnology
Environmental Science

Background:

Global and European Union policies advocate for replacing hazardous pesticides with safer alternatives to ensure sustainable food production.
Current research and development (R&D) for pesticide alternatives are insufficient and require expansion.

Purpose of the Study:

To explore the potential of RNA interference (RNAi) technology as a tool for reducing pesticide risks.
To highlight the need for broadening R&D efforts in the development of sustainable agricultural practices.

Main Methods:

This discussion reviews existing literature and policy documents.
It analyzes the capabilities of RNAi technology in the context of pesticide replacement.

Main Results:

RNAi-based technology presents a viable and efficient alternative for pest control.
The technology has the potential to significantly reduce reliance on conventional, contentious pesticides.

Conclusions:

RNAi technology can play a crucial role in achieving sustainable food production by mitigating pesticide risks.
Increased investment and research in RNAi are essential to meet global sustainability goals.