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Published on: December 5, 2020
A Universal Gelfoam 3-D Histoculture Method to Establish Patient-derived Cancer Cells (3D-PDCC) Without Fibroblasts
Jun Yamamoto1,2,3, Norihiko Sugisawa1,2, Kazuyuki Hamada1,2
1AntiCancer Inc, San Diego, CA, U.S.A.
Background/Aim:
The direct placement of patient tumors in 2-D culture on plastic or glass surfaces has inhibited the establishment of patient-derived cancer cells (PDCCs). The aim of the present study was to develop universal and efficient methods to prepare PDCCs.
Materials And Methods:
Fragments of patient-derived xenograft (PDX) tumors established form colon cancer liver metastasis (1 mm3) were placed on Gelfoam and cultured in DMEM.
Results:
PDX tumor fragments were cultured on Gelfoam. Cancer cells migrated from the explant and formed distinct 3-D structures in the Gelfoam. Each of the three PDCCs showed a distinct morphology. The cultures were essentially all cancer cells without fibroblasts, the opposite of what usually occurs in 2-D culture on plastic or glass. Gelfoam cultures could be readily passaged from one Gelfoam cube to anothers suggesting indefinite culture potential.
Conclusion:
A potentially universal method to establish PDCC using PDX tumors and 3-D Gelfoam histoculture was developed.
