Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cellulose and Pectic Polysaccharides

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator.

RSC chemical biology·2026

Same author

A green enzymatic route for the biotransformation of naphthalene to phthalic acid.

RSC chemical biology·2026

Same author

Influence of Metabolic Regulators on the Astaxanthin Synthesis by <i>Xanthophyllomyces dendrorhous</i> and the Transcriptome Analysis.

Journal of agricultural and food chemistry·2026

Same author

Microbial and Enzymatic Biodegradation of Polyurethane: From Depolymerization to Monomer Valorization.

Biotechnology journal·2025

Same author

Strategies to Improve the Efficiency of Enzymatic Carbon Dioxide Conversion <i>In Vitro</i>.

ACS synthetic biology·2025

Same author

Coproduction of 2-O-α-d-Glucosyl Glycerol and D-Allulose from Sucrose and Glycerol by Dual-Enzyme Cascade.

Journal of agricultural and food chemistry·2025

Search research articles

Related Experiment Video

Updated: Nov 26, 2025

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

Published on: June 17, 2014

Engineered LPMO Significantly Boosting Cellulase-Catalyzed Depolymerization of Cellulose.

Chao Cheng^1,2, Junaid Haider^2,3,4, Pi Liu^2,4

¹State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, P. R. China.

Journal of Agricultural and Food Chemistry

|December 8, 2020

Summary

This summary is machine-generated.

Directed evolution enhanced a lytic polysaccharide monooxygenase (LPMO) from Hahella chejuensis by substituting N526S in its cellulose-binding module (CBM). This modification significantly boosted crystalline cellulose degradation and enzyme activity for biorefinery applications.

Keywords:

cellulose binding module (CBM)cellulose degradation directed evolution lytic polysaccharide monooxygenase (LPMO)oxidative cleavage protein engineering

More Related Videos

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Related Experiment Videos

Last Updated: Nov 26, 2025

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

Published on: June 17, 2014

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Area of Science:

Biotechnology
Enzymology
Biorefining

Background:

Lytic polysaccharide monooxygenases (LPMOs) are key enzymes for cellulose depolymerization.
Enhancing LPMO activity is critical for developing efficient second-generation biorefineries.

Purpose of the Study:

To improve the activity and cellulose-binding capacity of *Hc*LPMO10 through protein engineering.
To investigate the role of the cellulose-binding module (CBM) in LPMO catalytic function.

Main Methods:

Directed evolution was used to identify beneficial mutations in *Hc*LPMO10.
Enzyme activity, H2O2 production, and cellulose binding were quantified.
Molecular modeling and molecular dynamics simulations were employed for structural analysis.

Main Results:

A beneficial N526S substitution was identified in the CBM of *Hc*LPMO10.
The engineered variant (M1) showed 1.9- to 2.7-fold higher activity and binding capacity compared to wild type.
M1 exhibited 2.1-fold higher synergistic activity with cellulase for crystalline cellulose degradation.

Conclusions:

The N526S substitution in the CBM enhances *Hc*LPMO10's binding to cellulose, increasing its catalytic efficiency.
The CBM plays a crucial role in the overall catalytic function of LPMOs.
This engineered LPMO holds promise for advanced biorefinery processes.