Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Signal Sequences and Sorting Receptors01:41

Signal Sequences and Sorting Receptors

12.8K
Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
12.8K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

7.9K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HDA19-mediated deacetylation of histone H3.3 at lysines 27 and 36 regulates plant sensitivity to salt stress.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Time-resolved spectroscopy of a photoactive dinuclear W/Ru complex: spectroscopic evidence for a metastable intermediate with side-on coordinated carbonyl ligand.

Chemical science·2026
Same author

Non-Statistical Assembly of Donor-Acceptor Cages for Light-Induced Charge Separation.

Angewandte Chemie (International ed. in English)·2026
Same author

Interactome Profiling of a Lysine Deacetylase Trapping Probe Library Uncovers Crosstalk Between HDAC6 and NF-κB Signaling.

Angewandte Chemie (International ed. in English)·2025
Same author

Multi-mass velocity-resolved kinetics of surface reactions at 100 kHz acquisition rate.

The Review of scientific instruments·2025
Same author

Cooperativity of Electron Transfer Coupled Spin Transitions in a Tetranuclear Fe/Co Prussian Blue Analogue Revealed by Ultrafast Spectroscopy.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Nov 26, 2025

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

11.0K

Engineered Sortases in Peptide and Protein Chemistry.

Christian Freund1, Dirk Schwarzer2

  • 1Freie Universität Berlin, Institute of Chemistry and Biochemistry, Thielallee 63, 14195, Berlin, Germany.

Chembiochem : a European Journal of Chemical Biology
|December 8, 2020
PubMed
Summary

Directed evolution created improved sortase enzymes for protein chemistry. These engineered sortases (Sortase A variants) show enhanced catalytic activity and new substrate specificities for sortase-mediated ligation (SML).

Keywords:
protein bioconjugationprotein engineeringprotein semisynthesissortasestranspeptidases

More Related Videos

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

25.1K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.5K

Related Experiment Videos

Last Updated: Nov 26, 2025

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

11.0K
Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

25.1K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Sortase A (SrtA) from Staphylococcus aureus anchors surface proteins to peptidoglycan via the LPxTG motif.
  • This transpeptidase activity is utilized for sortase-mediated ligation (SML) to join peptides and proteins.
  • Limitations in native Sa-SrtA efficiency and specificity necessitate improved biocatalysts.

Purpose of the Study:

  • To review recent advancements in directed evolution of sortase enzymes.
  • To highlight the development of novel sortase variants with enhanced catalytic properties.
  • To discuss applications of engineered sortases in biochemistry.

Main Methods:

  • Screening of sortase A variants from diverse microorganisms.
  • Directed protein evolution of the native Sa-SrtA enzyme.
  • Development of novel display platforms and screening formats for mutant libraries.

Main Results:

  • Isolation of sortase variants with significantly increased catalytic activity.
  • Identification of engineered sortases capable of recognizing new sorting motifs.
  • Demonstration of improved biocatalysts for sortase-mediated ligation.

Conclusions:

  • Directed evolution is a powerful strategy for engineering sortase enzymes.
  • Tailor-made sortases offer enhanced capabilities for SML applications.
  • These improved biocatalysts advance protein chemistry and biochemical research.