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Updated: Nov 26, 2025

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
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Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media.

Blendi Ura1, Fulvio Celsi1, Luisa Zupin1

  • 1Institute for Maternal and Child Health-IRCCS "Burlo Garofolo", 65/1 Via dell'Istria, 34137 Trieste, Italy.

International Journal of Molecular Sciences
|December 9, 2020
PubMed
Summary

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This summary is machine-generated.

Recurrent acute otitis media (RAOM) and adenotonsillar hypertrophy (ATH) in children involve distinct protein changes in adenoids. This proteomic study identified key pathways like neutrophil degranulation and glycolysis implicated in these common pediatric conditions.

Area of Science:

  • Pediatric Otolaryngology
  • Proteomics
  • Molecular Biology

Background:

  • Recurrent acute otitis media (RAOM) affects children, defined by multiple ear infection episodes.
  • Adenotonsillar hypertrophy (ATH) is linked to obstructive sleep apnea in children.
  • Understanding shared and distinct molecular mechanisms in RAOM and ATH is crucial for pediatric care.

Purpose of the Study:

  • To compare the proteomic profiles of adenoids from children with RAOM and ATH.
  • To identify differentially modulated proteins and associated biological pathways in these conditions.

Main Methods:

  • Proteomic analysis of adenoid tissue from 14 pediatric patients (RAOM and ATH).
  • Utilized two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) for protein identification.
Keywords:
2-DEadenotonsillar hypertrophyproteomicsrecurrent otitis

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  • Statistical analysis to determine significant protein expression changes (p-value < 0.05).

    • Main Results:

    • Identified 23 protein spots with significant expression differences between RAOM and ATH.
    • Detected alterations in proteins associated with neutrophil degranulation pathways.
    • Observed changes in proteins linked to glycolysis, suggesting metabolic pathway involvement.

    Conclusions:

    • Proteomic analysis reveals distinct protein expression patterns in adenoids of children with RAOM and ATH.
    • Neutrophil degranulation and glycolysis pathways are significantly modulated in these pediatric conditions.
    • Findings provide insights into the molecular underpinnings of RAOM and ATH, potentially guiding future therapeutic strategies.