Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.7K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.7K
Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation02:24

Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation

8.6K
Introduction
Like alkenes, alkynes can be reduced to alkanes in the presence of transition metal catalysts such as Pt, Pd, or Ni. The reaction involves two sequential syn additions of hydrogen via a cis-alkene intermediate.
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida.

Nature communications·2025
Same author

A highly active Burkholderia polyketoacyl-CoA thiolase for production of triacetic acid lactone.

Nature communications·2025
Same author

Real-Time Bioconjugation Reaction Monitoring of Antibody-Drug Conjugates with Multiattribute High-Throughput Hydrophobic Interaction Chromatography.

Analytical chemistry·2025
Same author

Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production in <i>Pseudomonas putida</i>.

Science advances·2025
Same author

Targeting Sialidase to PD1 Enhances T cell Function and Tumor Control.

ACS central science·2025
Same author

Discovery of acylsulfenic acid-featuring natural product sulfenicin and characterization of its biosynthesis.

Nature chemistry·2025

Related Experiment Video

Updated: Nov 26, 2025

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.3K

An automated workflow to screen alkene reductases using high-throughput thin layer chromatography.

Brett M Garabedian1,2, Corey W Meadows1,2, Florence Mingardon3

  • 1Joint BioEnergy Institute, 5885 Hollis Street, 4th floor, Emeryville, CA, 94608, USA.

Biotechnology for Biofuels
|December 9, 2020
PubMed
Summary

We developed an automated thin-layer chromatography (TLC) screening platform for enzyme engineering. This high-throughput method efficiently monitors geranylgeranyl reductase (SaGGR) activity and identifies promising enzyme variants.

Keywords:
AutomationGeranylgeranyl reductase (GGR)High-throughput screening (HTS)IsoprenoidsProtein engineeringThin layer chromatography (TLC)

More Related Videos

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
11:58

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

Published on: April 30, 2018

6.9K
Optimization of Radiochemical Reactions using Droplet Arrays
10:54

Optimization of Radiochemical Reactions using Droplet Arrays

Published on: February 12, 2021

3.7K

Related Experiment Videos

Last Updated: Nov 26, 2025

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.3K
Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
11:58

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

Published on: April 30, 2018

6.9K
Optimization of Radiochemical Reactions using Droplet Arrays
10:54

Optimization of Radiochemical Reactions using Droplet Arrays

Published on: February 12, 2021

3.7K

Area of Science:

  • Biochemistry
  • Synthetic Biology
  • Enzyme Engineering

Background:

  • High-throughput screening is crucial for enzyme engineering in synthetic biology.
  • Existing methods like chromatography and mass spectrometry are costly and complex.
  • There is a need for accessible, rapid screening tools.

Purpose of the Study:

  • To develop an automated, 96-well platform for enzyme activity screening.
  • To utilize thin-layer chromatography (TLC) for monitoring enzyme function.
  • To assess the in vitro activity of geranylgeranyl reductase (SaGGR).

Main Methods:

  • An automated 96-well TLC platform was developed.
  • Enzyme products were detected using a chromophore after separation on silica plates.
  • A codon-saturation mutagenesis library of SaGGR was screened for farnesol reduction activity.

Main Results:

  • The TLC platform successfully monitored SaGGR activity.
  • It distinguished fourfold differences in enzyme activity among mutants.
  • Results were validated using Gas Chromatography-Mass Spectrometry (GC-MS).

Conclusions:

  • The developed TLC workflow enables efficient screening of polyprenyl reductase activity.
  • This method is adaptable for analyzing diverse enzyme libraries.
  • It offers a cost-effective and accessible alternative for enzyme engineering.