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TRPS1 and YAP1 Regulate Cell Proliferation and Drug Resistance of Osteosarcoma via Competitively Binding to the

Jinyu Zhang1, Xiang Ma1, Ruiqi Zhou1

  • 1Department of Orthopaedics, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming 650118, People's Republic of China.

Oncotargets and Therapy
|December 9, 2020
PubMed
Abstract

Insights

CircRNA TADA2A targets miR-129-5p, affecting osteosarcoma (OS) cell growth and drug resistance by interacting with TRPS1 and YAP1 proteins. This finding offers potential therapeutic strategies for OS treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • The yes-associated protein (YAP) and trichorhinophalangeal syndrome 1 (TRPS1) are implicated in cancer development.
  • Their specific roles in osteosarcoma (OS) pathogenesis require further elucidation.

Purpose of the Study:

  • To investigate the modulatory effects and interrelationship of TRPS1 and YAP1 in OS cells.
  • To explore the role of circTADA2A in regulating these proteins and impacting OS progression.

Main Methods:

  • Quantitative analysis of YAP1 and TRPS1 expression in OS cell lines.
  • Assessment of circTADA2A knockdown effects on OS cell proliferation, drug resistance (cisplatin/DDP), and target gene expression (TRPS1, YAP1, miR-129-5p).
  • In vivo studies using xenograft OS tumor mouse models.

Main Results:

  • TRPS1 and YAP1 were upregulated in OS cells, with differential expression patterns.
  • CircTADA2A knockdown inhibited OS cell proliferation and DDP resistance, increasing miR-129-5p while decreasing TRPS1 and YAP1.
  • CircTADA2A knockdown suppressed tumor growth in vivo and affected TRPS1 protein and p-YAP1 levels.

Conclusions:

  • CircRNA TADA2A acts as a molecular sponge for miR-129-5p.
  • TRPS1 and YAP1 competitively bind to miR-129-5p, influencing OS cell proliferation and drug resistance.
  • CircTADA2A plays a significant role in OS progression and chemoresistance.
Keywords:
circRNAdrug resistanceosteosarcomatrichorhinophalangeal syndrome 1yes-associated protein 1

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