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Related Experiment Video

Updated: Nov 26, 2025

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

CRISPR-Cas9-mediated 75.5-Mb inversion in maize.

Chris Schwartz¹, Brian Lenderts¹, Lanie Feigenbutz¹

¹Corteva Agriscience, Johnston, IA, USA.

|December 10, 2020

Summary

This summary is machine-generated.

CRISPR-Cas9 gene editing technology was used to create a large chromosomal inversion in maize. This unlocks genetic variation for developing improved crop varieties.

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Area of Science:

Agricultural Science
Genetics
Molecular Biology

Background:

CRISPR-Cas9 is a versatile gene editing tool.
Its application has primarily focused on gene knockouts and deletions.
Targeted gene edits and insertions are less common but established uses.

Purpose of the Study:

To demonstrate the use of CRISPR-Cas9 for large-scale chromosomal rearrangements.
To induce a targeted pericentric inversion in maize chromosome 2.
To explore new avenues for genetic variation and crop improvement.

Main Methods:

Utilized CRISPR-Cas9 gene editing technology.
Targeted a 75.5-Mb pericentric inversion on chromosome 2 in elite maize.
Applied the technology in one of Corteva Agriscience's elite maize inbred lines.

Main Results:

Successfully mediated a targeted 75.5-Mb pericentric inversion in maize chromosome 2.
The induced inversion unlocked a significant chromosomal region.
This region now exhibits increased potential for genetic recombination.

Conclusions:

CRISPR-Cas9 can be effectively employed to engineer large chromosomal inversions.
This technique provides a novel strategy to access and utilize genetic variance.
Facilitates the development of new maize varieties with enhanced phenotypes.