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Expression of Hypoxia-Inducible Factor-1α (HIF1A) and Lp-PLA2 in Low, Intermediate, and High Cardiovascular Disease

Teuku Heriansyah1, Indah Nur Chomsy2, Kumboyono Kumboyono3

  • 1Department of Cardiology and Vascular Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.

Vascular Health and Risk Management
|December 10, 2020
PubMed

Insights

This study reveals a strong correlation between hypoxia-inducible factor-1α (HIF1A) and lipoprotein-associated phospholipase A2 (Lp-PLA2) in cardiovascular disease (CVD) risk populations. HIF1A expression rises with CVD risk, while Lp-PLA2 levels decrease, despite their strong association.

Area of Science:

  • Cardiovascular Research
  • Molecular Biology
  • Biochemistry

Background:

  • Cardiovascular disease (CVD) pathogenesis involves complex, multifactorial processes, primarily atherosclerosis.
  • Inflammation during atherogenesis can lead to hypoxia, activating hypoxia-inducible factor-1α (HIF1A).
  • Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an inflammatory mediator implicated in atherogenesis.

Purpose of the Study:

  • To quantify hypoxia-inducible factor-1α (HIF1A) expression.
  • To assess the correlation between HIF1A and Lp-PLA2 expression.
  • To analyze these markers across low-risk, intermediate, and high-risk cardiovascular disease (CVD) populations.

Main Methods:

  • Correlational analysis of 160 individuals categorized by Framingham Risk Score (FRS).
  • Measurement of Lp-PLA2 and HIF-1α concentrations in venous blood samples using ELISA.
  • Statistical analysis including normality, homogeneity, ANOVA, post hoc-Tukey HSD, and Pearson correlation.

Main Results:

  • A very strong positive correlation was observed between HIF1A and Lp-PLA2 expression across all risk groups (low-risk r=0.512, intermediate r=0.512, high-risk r=0.715, P<0.05).
  • HIF1A concentration increased significantly with higher CVD risk.
  • Lp-PLA2 concentrations did not consistently align with the Framingham Risk Score.

Conclusions:

  • HIF1A expression demonstrates an upward trend with increasing atherosclerosis risk.
  • Lp-PLA2 expression shows a decreasing trend as atherosclerosis risk, based on FRS, increases.
  • A significant correlation exists between HIF1A and Lp-PLA2 expression, irrespective of the FRS category.
Abstract

Keywords:
Framingham Risk ScoreHIF1ALp-PLA2atherosclerosiscardiovascular disease

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