Structure-function relationship analysis of fucoidan from sea cucumber (Holothuria tubulosa) on ameliorating...

The structure-function relationships of sea cucumber fucoidan has been seldom investigated. In this study, the effect of fucoidan which extracted from sea cucumber Holothuria tubulosa (Ht-FUC) with different molecular weight and chain conformation (Ht1/2/3/4) in metabolic inflammation was investigated. A co-cultured system comprising adipocytes and macrophages was used to explore the impact in vitro. The high-fat high-sucrose diet (HFD)-fed obese mice model was established to verified the effect of Ht-FUC in vivo. The results demonstrated that all examined Ht-FUC attenuated the productions of inflammatory cytokines, promoted the M2 phenotypic polarization of co-cultured macrophage by activating PPARγ. Furthermore Ht-FUC impressed lipolysis of the co-cultured adipocytes by inhibiting TLR4/NF-κB-dependent pathway. In vivo, Ht-FUC especially reduced serum inflammation level, attenuated M1/M2 polarization of liver Kupffer cells, and attenuated inflammatory infiltration of epididymal adipose tissue. Consistently, Ht3 played the best effect, and may be more beneficial for the intervention of metabolic disease. PRACTICAL APPLICATIONS: In this study, the structure-function relationships of fucoidan extracted from Holothuria tubulosa (Ht-FUC) on ameliorating metabolic inflammation was investigated for the first time. In this research, we reported that Ht-FUC ameliorating metabolic inflammation by improving FFA-induced macrophage polarization and lipolysis in both co-cultured system and obese mice. The study provides some theoretical basis for the high-value utilization of Ht-FUC with different molecular weight.