Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

8.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ciclopirox suppresses melanoma growth by activating ER stress-driven apoptosis and disrupting Src/STAT3 signaling.

Acta biochimica et biophysica Sinica·2026
Same author

Integrated micro-solid-phase extraction with miniature mass spectrometry for on-site detection of illicit drugs in human hair.

Journal of chromatography. A·2026
Same author

Tissue Heterogeneity-Driven Parallel Acquisition for High-Coverage MS/MS Imaging of Lipids.

Angewandte Chemie (International ed. in English)·2026
Same author

Enhanced identification of medicinal caterpillar fungus with structural lipidomic analysis.

Talanta·2026
Same author

Determination of <i>R</i>/<i>S</i>-enantiomers of methamphetamine and amphetamine in human hair with chiral stationary phase LC-MS/MS.

Bioanalysis·2026
Same author

<sup>13</sup>C-Isotope Tracing Structural Lipidomics for Resolving Phospholipid Metabolism Dynamics in Human Breast Cancer Cells.

Analytical chemistry·2026

Related Experiment Video

Updated: Nov 26, 2025

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.4K

High-Throughput Therapeutic Antibody Interference-Free High-Resolution Mass Spectrometry Assay for Monitoring

Rasa Santockyte1, Oscar Puig1, Naiyu Zheng1

  • 1Department of Translational Medicine, Bristol Myers Squibb, Route 206 and Province Line Road, Princeton, New Jersey 08543, United States.

Analytical Chemistry
|December 10, 2020
PubMed
Summary

A new high-throughput mass spectrometry method accurately measures monoclonal proteins (M-proteins) in multiple myeloma patients, overcoming limitations of current tests and resolving therapeutic antibody interference for improved diagnosis and monitoring.

More Related Videos

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

8.9K
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.7K

Related Experiment Videos

Last Updated: Nov 26, 2025

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.4K
An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

8.9K
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.7K

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Monoclonal protein (M-protein) measurement is crucial for multiple myeloma diagnosis and treatment monitoring.
  • Current assays lack sensitivity and are prone to interference from therapeutic antibodies (t-mAbs).
  • Previous mass spectrometry methods (miRAMM) showed promise but lacked sufficient throughput.

Purpose of the Study:

  • To develop a high-throughput liquid chromatography-high-resolution mass spectrometry (HT-LC-HRMS) method for sensitive and accurate M-protein quantification.
  • To resolve interference issues caused by therapeutic antibodies in M-protein measurements.
  • To improve the throughput of mass spectrometry-based M-protein analysis for clinical applications.

Main Methods:

  • Developed a fully automated immunocapture step integrated with HT-LC-HRMS.
  • Employed simplified chromatography and high-throughput data processing with automatic spectral deconvolution.
  • Utilized deconvoluted peak heights of M-protein and therapeutic antibody light chains for quantitation.

Main Results:

  • The HT-LC-HRMS method achieved a total measurement time of 11.0 minutes.
  • Successfully differentiated M-protein from elotuzumab in 98.9% of multiple myeloma serum samples.
  • Demonstrated a 25-fold improvement in sensitivity compared to immunofixation electrophoresis.

Conclusions:

  • The developed HT-LC-HRMS assay provides a sensitive, specific, and high-throughput solution for M-protein measurement.
  • This method effectively resolves therapeutic antibody interference, enhancing diagnostic and monitoring capabilities.
  • Offers potential for objective tracking of M-proteins, particularly in patients achieving complete response.