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Updated: Nov 26, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Sequencing of Complete Chloroplast Genomes
1Department of Genetics, Austrian Federal Research Centre for Forests (BFW), Vienna, Austria. berthold.heinze@bfw.gv.at.
Abstract:
In this chapter, frequently used methods for elucidating sequence and structure of chloroplast genomes are reviewed, as a current best practice guide. This concerns methods for DNA extraction, sequencing library preparation, and bioinformatics (assembly, verification, annotation, and sequence comparisons). Recommendations for standard data reporting practices are given-chloroplast genome sequencing reports can be highly formalized, and publication in the form of standard data reports is the best option for comparison and meta-analysis purposes.
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