Magnetically-driven drug and cell on demand release system using 3D printed alginate based hollow fiber scaffolds

Local delivery of drugs, proteins and living cells with on demand release manner using porous scaffolds has been widely used in the field of tissue engineering and cancer therapies. Drugs directly loaded in the porous scaffolds, are generally prone to free diffuse especially for long term incubation. Herein, in this study, hollow fiber alginate/iron oxide nanoparticles scaffolds were prepared by coaxial 3D printing with drugs, protein or living cells encapsulating in the core part (low concentration of alginate gels). Magnetically-driven on demand release was realized by extruding the loaded drugs, proteins and cells from the core part of the hollow fibers due to the deformation of the scaffolds under magnetic field. Additionally, the hollow fibers could sever as diffusion barriers to reduce uncontrolled diffusion of drugs, proteins and cells from scaffolds in the conditions of no required stimulation. The factors influencing the deformation of the scaffolds, as well as the release behavior were investigated. The data indicated that the scaffolds prepared by 10 wt% of alginate with 13% of iron oxide nanoparticles after crosslinking using 0.1 M CaCl₂ solution for 10 s showed repeated on demand release capability in vitro and in vivo under intermittently magnetic stimulation. Thus, this 3D printed alginate/iron oxide nanoparticles hollow scaffolds with on demand controlled delivery capability may prove useful for tissue engineering and disease therapies.