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Related Concept Videos

Gas Chromatography–Mass Spectrometry (GC–MS)01:14

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Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
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The peppermint breath test: a benchmarking protocol for breath sampling and analysis using GC-MS.

M Wilkinson1, I White2, K Hamshere1

  • 1Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.

Journal of Breath Research
|December 10, 2020
PubMed
Summary
This summary is machine-generated.

Standardizing breath analysis is crucial for disease diagnosis. This study established benchmark values for volatile organic compounds (VOCs) from peppermint, aiding thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) performance assessment.

Keywords:
benchmarkingbreathomicspeppermintstandardisation

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Respiratory Medicine

Background:

  • Exhaled breath volatile organic compounds (VOCs) offer diagnostic potential but vary in analysis methods.
  • Standardization is needed for reliable meta-analyses and confidence in breath research findings.
  • The Peppermint Initiative aims to standardize breath analysis protocols.

Purpose of the Study:

  • To establish benchmark values for thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) analysis of breath VOCs.
  • To assess inter-group performance using peppermint-derived VOCs.
  • To provide initial data for standardizing breath sample analysis.

Main Methods:

  • Headspace analysis of peppermint oil capsules to identify target VOCs.
  • Recruitment of participants across three European research groups.
  • Collection of breath samples post-peppermint ingestion following a standardized protocol.
  • Analysis of breath samples using TD-GC-MS by each research group.

Main Results:

  • Sixteen peppermint-derived VOCs were identified in breath samples.
  • 2,3-dehydro-1,8-cineole was identified as a potential metabolite.
  • Five key VOCs (α-pinene, β-pinene, eucalyptol, menthol, menthone) were quantified by all groups.
  • Benchmark washout times were determined for these five VOCs, with variations observed between groups.

Conclusions:

  • Initial benchmark values for TD-GC-MS analysis of breath VOCs were successfully generated.
  • These benchmarks aid in assessing the performance of breath analysis systems.
  • Further data from more groups will refine these benchmarks for improved standardization.