Related Experiment Video
Updated: Nov 26, 2025
11:20
Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
13.3K
Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells
Svetlana Rezinciuc1, Zhixin Tian2, Si Wu2
1Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Viruses
|December 11, 2020
Summary
This study introduces a new mass spectrometry method, HiPTMap, to map histone posttranslational modifications (PTMs) in CD8 T cells. HiPTMap reveals significant changes in histone PTMs during influenza infection, impacting T cell function.
Area of Science:
- Immunology
- Proteomics
- Epigenetics
Background:
- T cell function relies on epigenetic regulation, including histone posttranslational modifications (PTMs).
- Traditional mass spectrometry (MS) methods have limitations in comprehensively identifying histone PTMs.
- Understanding dynamic PTM changes is crucial for T cell responses.
Purpose of the Study:
- To develop and validate a novel mass spectrometry approach for comprehensive histone proteoform analysis.
- To identify and quantify histone PTMs in CD8 T cells during influenza infection.
- To investigate the impact of infection and T cell activation on histone modifications.
Main Methods:
- Utilized online two-dimensional liquid chromatography-tandem MS (2D LC-MS/MS) with alternating electron transfer dissociation (ETD) and collision-induced dissociation (CID).
- Employed reversed-phase liquid chromatography (RPLC) and weak cation exchange hydrophilic interaction liquid chromatography (WCX-HILIC) for separation.
- Developed the histone-intact protein PTM mapping (HiPTMap) strategy for proteoform identification and quantification.
Main Results:
- HiPTMap successfully identified and quantified histone proteoforms in CD8 T cells.
- Influenza infection significantly altered histone PTMs, with changes observed during T cell migration.
- Responding T cells exhibited increased transcription-enhancing modifications during secondary infections.
Conclusions:
- The HiPTMap method provides a powerful tool for deep profiling of histone PTMs.
- Dynamic changes in histone PTMs are critical for CD8 T cell responses to viral infections.
- Epigenetic reprogramming of T cells is essential for effective immunity.
Related Concept Videos
01:32Chromatin Modification in iPS Cells
2.0K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.0K
02:32Histone Modification
15.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.2K
01:29Immune Response Against Viral Pathogens
1.3K
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
1.3K
02:25Spreading of Chromatin Modifications
9.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
9.0K
02:04Covalently Linked Protein Regulators
8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.2K