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Plasma miR-193a-3p can be a potential biomarker for the diagnosis of diabetic nephropathy
Yan Hong1,2, Jidong Wang1,2, Lai Zhang1,2
1Department of Nephrology, the Affiliated Wuxi No.2 People's Hospital of Nanjing Medical University, Wuxi, China.
Background:
Diabetic nephropathy is one of the most common microvascular complications in patients with diabetes. MicroRNA (miRNA, miR) is closely related to the formation, development and pathophysiology of diabetic nephropathy. We aimed to investigate whether miR-193a-3p could be used as a potential biomarker for the diagnosis of diabetic nephropathy.
Methods:
Plasma samples were collected from all the participants. TaqMan Low Density Array analysis was employed to obtain the miRNA profiles of plasma samples, and qRT-PCR was used to confirm the result. Receiver operating characteristic curves were employed to evaluate the specificity and sensitivity of miR-193a-3p for predicting diabetic nephropathy.
Results:
The expression of miR-193a-3p and miR-320c was elevated and miR-27a-3p was decreased in diabetic nephropathy patients compared to patients with type 2 diabetes and healthy controls. We found that, in diabetic nephropathy patients, the elevated miR-193a-3p expression had a negative correlation with the level of evaluate glomerular filtration rate, while a positive correlation with the level of proteinuria. We further demonstrated that miR-193a-3p could be employed to distinguish patients with diabetic nephropathy. The Kaplan-Meier analysis showed that the high expression of miR-193a-3p significantly shortened the dialysis-free survival of diabetic nephropathy patients.
Conclusion:
In conclusion, miR-193a-3p is involved in diabetic nephropathy pathogenesis and may serve as a potentially novel diagnostic biomarker for diabetic nephropathy.
Insights
MicroRNA-193a-3p (miR-193a-3p) is elevated in diabetic nephropathy patients and correlates with disease severity. This microRNA shows potential as a novel diagnostic biomarker for diabetic kidney disease.
Area of Science:
- Nephrology
- Endocrinology
- Molecular Biology
Background:
- Diabetic nephropathy is a common complication of diabetes, affecting kidney microvasculature.
- MicroRNAs (miRNAs) play a role in the development and pathophysiology of diabetic nephropathy.
- Identifying reliable biomarkers for early diagnosis is crucial.
Purpose of the Study:
- To investigate the potential of miR-193a-3p as a diagnostic biomarker for diabetic nephropathy.
- To assess the correlation between miR-193a-3p expression and clinical parameters in diabetic nephropathy patients.
Main Methods:
- Plasma samples were analyzed for miRNA profiles using TaqMan Low Density Array and qRT-PCR.
- Receiver operating characteristic (ROC) curves were used to evaluate diagnostic accuracy.
- Kaplan-Meier analysis assessed the impact of miR-193a-3p on patient survival.
Main Results:
- miR-193a-3p expression was significantly elevated in diabetic nephropathy patients compared to controls.
- Elevated miR-193a-3p correlated negatively with glomerular filtration rate and positively with proteinuria.
- miR-193a-3p demonstrated diagnostic potential and was associated with reduced dialysis-free survival.
Conclusions:
- miR-193a-3p is implicated in the pathogenesis of diabetic nephropathy.
- miR-193a-3p may serve as a novel and promising diagnostic biomarker for diabetic nephropathy.
- Further research is warranted to validate its clinical utility.
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