Jove
Visualize
Contact Us

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Stacked Generalization of 3D Orthogonal Deep Learning Convolutional Neural Networks for Improved Detection of White Matter Hyperintensities in 3D FLAIR Images.

AJNR. American journal of neuroradiology·2021
Same author

Fully Automated Segmentation of Globes for Volume Quantification in CT Images of Orbits using Deep Learning.

AJNR. American journal of neuroradiology·2020
Same author

Clinical Utility of a Novel Ultrafast T2-Weighted Sequence for Spine Imaging.

AJNR. American journal of neuroradiology·2018
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 26, 2025

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets
07:44

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets

Published on: July 16, 2021

2.4K

MR Imaging Characteristics of Intraocular Perfluoro-n-Octane.

M T Williams1, J E Williams2, B A Winegar3

  • 1From the Departments of Ophthalmology and Vision Science (M.T.W., J.B.C.) mtwilli588@gmail.com.

AJNR. American Journal of Neuroradiology
|December 11, 2020
PubMed
Summary

Magnetic resonance imaging (MRI) reveals unique characteristics of intraocular perfluoro-n-octane, a tamponade liquid used in retinal detachment repair. Understanding these features is crucial to differentiate it from other intraocular abnormalities.

More Related Videos

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium
05:19

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

Published on: January 16, 2012

22.0K
Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

11.7K

Related Experiment Videos

Last Updated: Nov 26, 2025

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets
07:44

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets

Published on: July 16, 2021

2.4K
A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium
05:19

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

Published on: January 16, 2012

22.0K
Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

11.7K

Area of Science:

  • Ophthalmology
  • Radiology
  • Medical Imaging

Background:

  • Complex retinal detachment repair often requires intraocular tamponade agents.
  • Perfluoro-n-octane is a dense liquid utilized for both intraoperative and postoperative tamponade.
  • Accurate identification of intraocular substances is critical for patient management.

Observation:

  • Perfluoro-n-octane exhibits distinct signal intensities and patterns on various MRI sequences.
  • Its unique physical properties influence its appearance within the vitreous cavity.
  • Comparison with known intraocular pathologies aids in differentiating perfluoro-n-octane.

Findings:

  • The study details the specific MR imaging signatures of perfluoro-n-octane.
  • These characteristics allow for differentiation from other intraocular conditions, including tumors and foreign bodies.
  • Trace amounts of residual perfluoro-n-octane can mimic other abnormalities.

Implications:

  • Enhanced understanding of perfluoro-n-octane's MRI appearance improves diagnostic accuracy in ophthalmology.
  • Radiologists and ophthalmologists can better interpret postoperative imaging in patients who underwent retinal repair.
  • This knowledge helps prevent misdiagnosis and guides appropriate clinical decision-making.