Using Metabolic Engineering to Connect Molecular Biology Techniques to Societal Challenges.

Area of Science:

• Synthetic Biology
• Metabolic Engineering
• Biotechnology Education

Background:

• Genetically modified organisms (GMOs) are typically taught as single-gene modifications.
• Existing GMO education often focuses on crop traits, not complex metabolic pathways.
• There's a need for advanced modules on metabolic engineering and sustainable production.

Purpose of the Study:

• To introduce upper-division undergraduates and graduate students to metabolic engineering techniques in yeast.
• To connect laboratory techniques with the societal impact of sustainable product development.
• To foster student engagement with sustainability challenges through case study creation.

Main Methods:

• Utilized recombination-based methods for assembling metabolic pathways in Saccharomyces cerevisiae.
• Implemented genetic screens to identify yeast genes influencing metabolite yield.
• Employed error-prone PCR for optimizing metabolic pathway function.
• Integrated case studies on yeast-derived products (e.g., Impossible Burger) and a sustainability project.

Main Results:

• Students gained hands-on experience with metabolic engineering techniques.
• Students developed case studies on yeast metabolic engineering for sustainability, adaptable for future teaching.
• The module successfully connected laboratory work with societal and ethical considerations.

Conclusions:

• The developed 8-week laboratory module effectively teaches advanced metabolic engineering and sustainability concepts.
• Student-created case studies enhance peer learning and promote student ownership.
• The module and its sustainability project are adaptable for various biology and bioethics courses.