An efficient, high-throughput method for the simultaneous exposure of drought stress and bacterial infection in
Aanchal Choudhary1, Muthappa Senthil-Kumar1
1National Institute of Plant Genome Research Aruna Asaf Ali Marg New Delhi 110067 India.
Premise:
We developed a systematic protocol for the easy, high-throughput, qualitative, and quantitative assessment of the patho-morphological, physiological, and molecular responses of Arabidopsis thaliana plants simultaneously subjected to drought and bacterial infection. This approach will assist studies elucidating plant adaptation strategies to combat combined stresses.
Methods And Results:
Plants were grown in small screw-capped containers, individual pots, or pot strips. Watering was withheld from 30-day-old plants, which were subsequently infected with Pseudomonas syringae pv. tomato DC3000 using a dip inoculation. The natural development of both drought and bacterial infection was successfully and rapidly replicated in large numbers of plants, which is difficult to achieve with existing protocols.
Conclusions:
Our protocol offers a simple, low-cost, high-throughput strategy for the rapid and easy bacterial infection of large numbers of plants. It can be used in large-scale mutant and ecotype screenings under combined stresses and for other foliar pathogens in different plant species.
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