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Label-Free Near-Infrared Plasmonic Sensing Technique for DNA Detection at Ultralow Concentrations.

Shimeng Chen¹, Chuan Liu², Yun Liu³

¹School of Optoelectronic Engineering and Instrumentation Science Dalian University of Technology Dalian 116024 China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|December 11, 2020

Summary

This study presents a highly sensitive nanoplasmonic biosensing method for detecting low concentrations of biomolecules. The approach uses near-infrared light and gold nanotriangles to achieve ultrasensitive, label-free detection of single-stranded DNA (ssDNA).

Keywords:

biosensing nanostructure plasmonic sensors signal enhancement ultrasensitivity

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Published on: October 6, 2017

Area of Science:

Nanotechnology
Biosensing
Plasmonics
Biomolecular detection

Background:

Low-concentration biomolecular detection is crucial for early disease diagnosis and biological measurements.
Existing biosensing techniques often face limitations in sensitivity and detection limits for ultralow concentrations.

Purpose of the Study:

To demonstrate a highly sensitive nanoplasmonic biosensing approach for ultrasensitive biomolecular detection.
To achieve label-free detection of single-stranded DNA (ssDNA) at nanomolar levels and below.

Main Methods:

Utilized near-infrared (NIR) plasmonic excitation on a continuous gold-coated nanotriangular array.
Employed sharp nanotriangular metallic tips to localize plasmonic near-fields for enhanced molecular perception.
Investigated the coupling effect between nanotriangles and gold nanoparticles to improve detection limits.

Main Results:

Achieved high spectral sensitivity (42103.8 nm per RIU) and a figure of merit (367.812).
Demonstrated direct, label-free assay of ssDNA at nanomolar levels.
Recorded a significantly improved detection limit for ssDNA as 1.2 × 10^-18 m.

Conclusions:

The developed nanoplasmonic biosensing approach offers high bulk and surface sensitivities.
This method provides a simple and effective strategy for label-free ultralow-concentration biomolecular detection.
The findings represent a significant advancement in ultrasensitive sensing techniques for diagnostics.