Temperature and oxygen level determine N₂ O respiration activities of heterotrophic N₂ O-reducing bacteria:...

Nitrous oxide (N₂ O), a potent greenhouse gas, is reduced to N₂ gas by N₂ O-reducing bacteria (N₂ ORB), a process which represents an N₂ O sink in natural and engineered ecosystems. The N₂ O sink activity by N₂ ORB depends on temperature and O₂ exposure, yet the specifics are not yet understood. This study explores the effects of temperature and oxygen exposure on biokinetics of pure culture N₂ ORB. Four N₂ ORB, representing either clade I type nosZ (Pseudomonas stutzeri JCM5965 and Paracoccus denitrificans NBRC102528) or clade II type nosZ (Azospira sp. strains I09 and I13), were individually tested. The higher activation energy for N₂ O by Azospira sp. strain I13 (114.0 ± 22.6 kJ mol^-1 ) compared with the other tested N₂ ORB (38.3-60.1 kJ mol^-1 ) indicates that N₂ ORB can adapt to different temperatures. The O₂ inhibition constants (K_I ) of Azospira sp. strain I09 and Ps. stutzeri JCM5965 increased from 0.06 ± 0.05 and 0.05 ± 0.02 μmol L^-1 to 0.92 ± 0.24 and 0.84 ± 0.31 μmol L^-1 , respectively, as the temperature increased from 15°C to 35°C, while that of Azospira sp. strain I13 was temperature-independent (p = 0.106). Within the range of temperatures examined, Azospira sp. strain I13 had a faster recovery after O₂ exposure compared with Azospira sp. strain I09 and Ps. stutzeri JCM5965 (p < 0.05). These results suggest that temperature and O₂ exposure result in the growth of ecophysiologically distinct N₂ ORB as N₂ O sinks. This knowledge can help develop a suitable N₂ O mitigation strategy according to the physiologies of the predominant N₂ ORB.