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Integrin α11β1 is a receptor for collagen XIII
Jarkko Koivunen1, Hongmin Tu2, Antti Kemppainen1
1Oulu Center for Cell-Matrix Research, Faculty of Biochemistry and Molecular Medicine, University of Oulu, P.O. Box 5400, FIN-90014, Oulu, Finland.
Cell and Tissue Research
|December 11, 2020
Summary
Integrin α11β1 is identified as a novel collagen XIII receptor, revealing its crucial role in regulating bone homeostasis and tissue development.
Area of Science:
- Biochemistry
- Cell Biology
- Skeletal Biology
Background:
- Collagen XIII is a transmembrane collagen involved in tissue development and homeostasis.
- Integrins, such as integrin α11β1, are cell surface receptors that mediate cell-matrix interactions.
- Both collagen XIII and integrin α11β1 are implicated in bone homeostasis.
Purpose of the Study:
- To investigate whether integrin α11β1 functions as a receptor for collagen XIII.
- To identify the specific collagen XIII motifs recognized by integrin α11β1.
- To elucidate the in vivo role of the collagen XIII-integrin α11β1 interaction in bone homeostasis.
Main Methods:
- Utilized C2C12 cells engineered to express integrin α11β1.
- Employed surface plasmon resonance and pull-down assays to confirm interaction.
- Analyzed bone morphology in α11 null and collagen XIII overexpressing mice using microcomputed tomography.
Main Results:
- Confirmed integrin α11β1 binds to collagen XIII.
- Identified GPKGER and GF(S)QGEK as recognition sites for integrin α11β1.
- Observed significant bone abnormalities in mice with altered collagen XIII and integrin α11 levels, with double mutants showing a milder phenotype.
Conclusions:
- Integrin α11β1 is a novel receptor for collagen XIII.
- The collagen XIII-integrin α11β1 axis plays a significant role in maintaining bone homeostasis.
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