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Updated: Nov 26, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
Published on: March 31, 2022
Cas9 in Human Embryos: On Target but No Repair
1Danish National Research Foundation Center for Chromosome Stability, Department of Cellular and Molecular Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
Abstract:
In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for "gene surgery".
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