Related Experiment Video
Updated: Nov 26, 2025
Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses
Published on: January 17, 2025
Marmoset Brain Mapping V3: Population multi-modal standard volumetric and surface-based templates
Cirong Liu1, Cecil Chern-Chyi Yen2, Diego Szczupak3
1Department of Neurobiology, University of Pittsburgh Brain Institute, 3501 Fifth Avenue, 6065 Biomedical Science Tower 3, Pittsburgh PA, USA; Cerebral Microcirculation Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA; CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China.
New population-based brain templates for the common marmoset (Callithrix jacchus) enable advanced neuroimaging analysis. These tools reveal significant individual variations in brain structure, crucial for future research.
Area of Science:
- Neuroimaging
- Comparative Anatomy
- Primate Neuroscience
Background:
- Standard anatomical brain templates are essential for analyzing neuroimaging data across subjects.
- Previous common marmoset brain templates lacked essential functionalities and were often based on single individuals.
Purpose of the Study:
- To develop new population-based, in-vivo standard templates and analysis tools for the common marmoset brain.
- To establish a standardized coordinate system and versatile platform for neuroimaging and connectome studies.
Main Methods:
- Utilized multi-modal data from 27 common marmosets, including T1w, T2w, DTI, MRI, and CT images.
- Performed multi-atlas labeling and constructed accurate tissue-type segmentation maps.
- Developed brain surfaces and cortical flat maps compatible with standard neuroimaging software.
Main Results:
- Created new population-based in-vivo standard templates and associated analysis tools for the common marmoset.
- Generated highly accurate tissue-type segmentation maps and fully featured brain surfaces.
- Revealed significant population-level variations in marmoset brain shape, size, and regional volumes.
Conclusions:
- The new population-based template and tools offer a versatile platform for common marmoset neuroimaging and connectome research.
- These resources provide a standard coordinate system, facilitating comparative analyses and highlighting individual brain variability.
- Version 3 of the Marmoset Brain Mapping Project is publicly available, advancing the field of primate brain research.
