Immunosorbent assay based on upconversion nanoparticles controllable assembly for simultaneous detection of three...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Antibiotic abuse fuels the rise of resistant strains and superbugs, complicating human disease treatment.
• Existing detection methods for antibiotics may lack the sensitivity, speed, or throughput required for effective monitoring.

Purpose of the Study:

• To develop a highly sensitive and high-throughput assay for accurate, quantitative antibiotic detection.
• To address the challenges posed by antibiotic resistance and superbug development through improved diagnostics.

Main Methods:

• Development of an avidin-biotin complex upconversion nanoparticle controllable assembly immunosorbent assay (ABC-ULISA).
• Utilized streptavidin and biotin-labeled upconversion nanoparticles for signal amplification via avidin-biotin-upconversion complex formation.
• Employed a 96-well enzyme-linked plate for high-throughput detection with 808 nm excitation upconversion nanoparticles.

Main Results:

• Achieved sensitive, quantitative detection of three antibiotics: sulfamethazine (0.15 ng/mL), sarafloxacin (0.03 ng/mL), and tetracycline (0.05 ng/mL).
• Demonstrated significantly lower detection limits compared to traditional ELISA and ordinary ULISA.
• Confirmed the assay's versatility for detecting various targets by changing antibodies, with stable and reliable results.

Conclusions:

• The developed ABC-ULISA offers a sensitive, rapid, and high-throughput method for antibiotic detection.
• This versatile assay shows potential for miniaturization, commercialization, and on-site applications in combating antibiotic resistance.