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Contribution of PDGFRα lineage cells in adult mouse hematopoiesis

Asaka Miura1, Takashi Shimbo2, Tomomi Kitayama3

  • 1Department of Stem Cell Therapy Science, Graduate School of Medicine, Osaka University, Suita, 565-0871, Japan.

Biochemical and Biophysical Research Communications
|December 14, 2020
PubMed

Insights

Platelet-derived growth factor receptor alpha (PDGFRα)-positive cells are a significant component of adult hematopoietic cells in mice. These PDGFRα lineage-positive hematopoietic cells exhibit similar characteristics and functions to PDGFRα lineage-negative cells.

Area of Science:

  • Developmental Biology
  • Hematopoiesis Research
  • Cell Lineage Tracing

Background:

  • Platelet-derived growth factor receptor alpha (PDGFRα) marks mesodermal cells.
  • PDGFRα+ cells contribute to hematopoietic cells during mouse embryogenesis.
  • The role of PDGFRα+ hematopoietic cells in adult hematopoiesis remains unclear.

Purpose of the Study:

  • To characterize PDGFRα lineage-positive (PDGFRα Lin+) hematopoietic cells (HCs) in adult mice.
  • To investigate the contribution and characteristics of PDGFRα Lin+ HCs in bone marrow and peripheral blood.
  • To compare PDGFRα Lin+ HCs with PDGFRα Lin- HCs in adult hematopoiesis.

Main Methods:

  • Utilized PDGFRα-CRE; ROSAtdTomato reporter mice for lineage tracing.
  • Employed flow cytometry to quantify PDGFRα Lin+ HCs.
  • Conducted single-cell RNA-sequencing (scRNA-seq) to analyze transcriptomic profiles.

Main Results:

  • PDGFRα Lin+ HCs constitute approximately 20% of total HCs in adult mouse bone marrow and peripheral blood.
  • Myeloid and lymphoid subpopulations were similar between PDGFRα Lin+ and PDGFRα Lin- HCs.
  • scRNA-seq revealed overlapping transcriptomic signatures, suggesting equivalent functions.

Conclusions:

  • PDGFRα Lin+ HCs play a significant role in physiological adult hematopoiesis.
  • PDGFRα Lin+ and PDGFRα Lin- HCs appear functionally equivalent in adult mice.
  • Further studies are needed to explore the pathophysiological roles of PDGFRα Lin+ HCs.
Keywords:
Bone marrowHematopoietic cellMesodermPDGFRαSingle-cell RNA-sequencing

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