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Updated: Nov 26, 2025
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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
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Jekyll and Hyde: Activating the Hypoxic Translational Machinery.
J J David Ho1, Jonathan H Schatz1, Jim Uniacke2
1Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33136, USA; Division of Hematology, Department of Medicine, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Trends in Biochemical Sciences
|December 14, 2020
Summary
Hypoxia triggers a distinct protein synthesis system, remodeling the cell
Area of Science:
- Molecular Biology
- Cellular Adaptation
- Biochemistry
Background:
- Global translational remodeling is key for biological adaptation.
- Oxygen deficiency (hypoxia) profoundly impacts cellular processes.
- The hypoxia-inducible factor (HIF) transcriptional program is a known response to hypoxia.
Purpose of the Study:
- To elucidate the mechanisms of translational remodeling under hypoxic conditions.
- To investigate the activation of a specific hypoxic translational architecture.
- To differentiate hypoxic translation from mRNA-level changes.
Main Methods:
- Analysis of protein synthesis machinery under varying oxygen levels.
- Characterization of the 'Jekyll' (basal) and 'Hyde' (hypoxic) translational states.
- Comparison of translational changes with mRNA expression profiles.
Main Results:
- Hypoxia disables the basal protein synthesis machinery.
- A distinct hypoxic translational architecture ('Hyde') is activated.
- Translational remodeling occurs independently of mRNA fluctuations.
Conclusions:
- Hypoxic translational remodeling is a critical adaptive mechanism.
- This process involves a shift from basal to specialized translation.
- The 'Hyde' paradigm offers new insights beyond traditional HIF pathways.
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