Recent advances in chemical analysis of licorice (Gan-Cao)

Gan-Cao, or licorice, the dried roots and rhizomes of Glycyrrhiza uralensis, G.glabra, and G.inflata, has received considerable interest due to its extensive application in traditional Chinese medicine (TCM) prescriptions (60% approximately), clinical therapy, and as food additives world-wide. Chemical analysis is an important approach to understand the active pharmaceutical components in licorice and its prescriptions, as well as to develop novel methodologies for their quality assessment and control. This comprehensive review describes the advances in the chemical analysis, including sample preparation methods, qualitative and quantitative analysis and biological specimen analysis, based on 113 references for the recent years. Newly established methods are summarized, such as high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC-MS), capillary electrophoresis (CE) and near infrared spectroscopy (NIR), which allows the identification, authentication, and simultaneous detection of multiple compounds in licorice with higher throughput and sensitivity. It is anticipated that this review could provide imperative information for improving the existing quality evaluation methods of licorice and afford scientific basis for further researches on the pharmacodynamic substances of licorice.