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Dryocrassin ABBA ameliorates Streptococcus pneumoniae-induced infection in vitro through inhibiting Streptococcus

Hongfa Lv1, Tianqi Fang1, Fanrong Kong1

  • 1Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, Jilin, China.

Microbial Pathogenesis
|December 14, 2020
PubMed

Insights

Dryocrassin ABBA demonstrates a bactericidal effect against Streptococcus pneumoniae infections. It also inhibits pneumolysin (PLY) activity, reducing cell injury without cytotoxicity.

Area of Science:

  • Microbiology
  • Pharmacology

Background:

  • Streptococcus pneumoniae infections pose a significant public health threat.
  • Pneumolysin (PLY) is a key virulence factor contributing to S. pneumoniae pathogenesis.
  • Novel therapeutic strategies are needed to combat pneumococcal infections.

Purpose of the Study:

  • To investigate the in vitro efficacy of dryocrassin ABBA against Streptococcus pneumoniae.
  • To evaluate ABBA's mechanism of action, including its effects on bacterial viability and pneumolysin activity.

Main Methods:

  • Minimal inhibitory concentration, growth curve, hemolysis, LIVE/DEAD staining, and time-killing assays were employed.
  • Oligomerization inhibition, LDH release, and cytotoxicity assays assessed ABBA's impact on pneumolysin and host cells.

Main Results:

  • ABBA exhibited a bactericidal effect against S. pneumoniae at concentrations below 8 μg/ml.
  • ABBA inhibited pneumolysin oligomerization, reducing its hemolytic activity.
  • ABBA ameliorated S. pneumoniae-induced cell injury by neutralizing PLY activity without exhibiting cytotoxicity.

Conclusions:

  • Dryocrassin ABBA is a promising compound for treating S. pneumoniae infections.
  • ABBA exerts its therapeutic effect through direct bactericidal action and neutralization of pneumolysin.
  • ABBA presents a dual mechanism of action with potential for development as an anti-pneumococcal agent.
Keywords:
AntivirulenceDryocrassin ABBAOligomerizationPneumolysinStreptococcus pneumoniae

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