Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Identification of proteins exhibiting <i>in vitro</i> RNA chaperone activity through gradient profiling in the Lyme disease spirochete, <i>Borrelia burgdorferi</i>.

RNA biology·2026

Same author

The <i>Borreliella burgdorferi</i> BosR-associated small non-coding RNA BasA regulates virulence.

Infection and immunity·2026

Same author

Making the best of a sticky situation: Infection-mediated endothelial activation promotes new interactions with adhesins of the host-adapted Lyme disease spirochete.

bioRxiv : the preprint server for biology·2026

Same author

Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1.

Microbiology spectrum·2024

Same author

Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1.

bioRxiv : the preprint server for biology·2024

Same author

Structure analysis of the telomere resolvase from the Lyme disease spirochete Borrelia garinii reveals functional divergence of its C-terminal domain.

Nucleic acids research·2024

Search research articles

Related Experiment Video

Updated: Nov 26, 2025

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Published on: October 16, 2014

Live Imaging.

George Chaconas¹, Tara J Moriarty², Jon Skare³

¹Department of Biochemistry and Molecular Biology and Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Alberta, T2N 4N1, Canada.

Current Issues in Molecular Biology

|December 14, 2020

Summary

Live imaging techniques allow visualization of Borrelia spirochetes during host interactions. This review covers methods like whole animal imaging and intravital microscopy, highlighting their impact on spirochete biology.

More Related Videos

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Published on: July 27, 2010

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Related Experiment Videos

Last Updated: Nov 26, 2025

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Published on: October 16, 2014

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Published on: July 27, 2010

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Area of Science:

Microbiology
Infectious Diseases
Microscopy

Background:

Visualizing pathogens during host interactions offers unique insights.
Live imaging provides data unobtainable through other methods.
Borrelia spirochetes are pathogens requiring advanced visualization techniques.

Purpose of the Study:

To review primary modalities for live imaging of Borrelia spirochetes.
To discuss the strengths, weaknesses, and optimal uses of each imaging method.
To highlight the impact of live imaging on understanding spirochete biology.

Main Methods:

Whole animal imaging
Intravital microscopy
Live cell imaging

Main Results:

Each method offers distinct advantages and limitations for Borrelia visualization.
Live imaging has significantly advanced the study of spirochete biology.
A collection of images showcasing advancements in the field is presented.

Conclusions:

Live imaging is a powerful and relatively recent development in Borrelia research.
The reviewed methods provide critical tools for studying pathogen-host interactions.
Continued application of live imaging will further elucidate spirochete biology and pathogenesis.