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[Total serum IgE: normal pediatric values using the FAST IgE fluoroimmunoenzymatic technic]
X H Nguyen-Thi1, M H Chabane, M Lafay
1Laboratoire de Biochimie, Hôpital Herold, Paris.
Allergie Et Immunologie
|September 1, 1986
Summary
A new fluorescence enzyme immunoassay, FAST IgE, accurately measures total immunoglobulin E (IgE) in children. This method is sensitive for detecting both low and high IgE levels in pediatric patients.
Area of Science:
- Clinical immunology
- Pediatric diagnostics
- Immunoassay technology
Context:
- Accurate measurement of total immunoglobulin E (IgE) is crucial for diagnosing allergic diseases in children.
- Existing methods may have limitations in sensitivity or applicability to pediatric serum samples.
- The development of novel diagnostic tools is essential for improving pediatric healthcare.
Purpose:
- To evaluate the application of a new direct, sandwich-type fluorescence enzyme immunoassay (FAST IgE) for total IgE determination in pediatrics.
- To establish reference ranges for total IgE in healthy children across different age groups using the FAST IgE assay.
- To assess the assay's sensitivity and reliability for detecting both normal and pathological IgE levels in infants and young children.
Summary:
- A novel fluorescence enzyme immunoassay, FAST IgE, was utilized for pediatric total IgE determination.
- The assay demonstrated good sensitivity for quantifying low normal and high pathological IgE levels in infants and children.
- Reference values for total IgE were established in healthy children aged 0-14 years, stratified into seven age groups.
Impact:
- Provides a sensitive and reliable method for pediatric total IgE measurement.
- Facilitates accurate diagnosis and monitoring of allergic conditions in children.
- Establishes age-specific reference ranges, improving clinical interpretation of IgE levels in pediatric populations.