RNA-binding protein SORBS2 suppresses clear cell renal cell carcinoma metastasis by enhancing MTUS1 mRNA stability

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Updated: Nov 26, 2025

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

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Qi Lv1, Fan Dong2, Yong Zhou3

  • 1Department of Medical Imaging, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Cell Death & Disease
|December 14, 2020
PubMed

Insights

Sorbin and SH3 domain-containing 2 (SORBS2) suppresses kidney cancer metastasis by increasing microtubule-associated tumor-suppressor 1 (MTUS1) mRNA stability. This RNA-binding protein enhances MTUS1 expression, inhibiting cancer cell spread.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • RNA-binding proteins (RBPs) are crucial in posttranscriptional gene regulation and cancer progression.
  • Sorbin and SH3 domain-containing 2 (SORBS2) functions as a tumor suppressor in various cancers.
  • Decreased SORBS2 expression correlates with metastasis and poorer survival in kidney cancer.

Purpose of the Study:

  • To investigate the role of SORBS2 in clear cell renal cell carcinoma (ccRCC) metastasis.
  • To elucidate the molecular mechanisms by which SORBS2 affects ccRCC progression.

Main Methods:

  • Integrative analysis of clinical specimens.
  • Overexpression studies in ccRCC cell lines.
  • Transcriptome-wide analysis to identify SORBS2 targets.
  • RNA immunoprecipitation and mRNA stability assays.
  • Western blotting and phosphorylation analysis.

Main Results:

  • SORBS2 expression is significantly reduced in metastatic ccRCC tissues and linked to overall survival.
  • Overexpressing SORBS2 reduces metastatic potential in ccRCC cells.
  • SORBS2 directly binds to the 3' untranslated region of MTUS1 mRNA, enhancing its stability.
  • MTUS1 promotes KIF2C phosphorylation, influencing microtubule dynamics.

Conclusions:

  • SORBS2 acts as a suppressor of ccRCC metastasis by stabilizing MTUS1 mRNA.
  • The SORBS2-MTUS1 axis offers a novel therapeutic target for ccRCC.
  • Understanding RBP functions provides new insights into ccRCC progression.

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