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Updated: Nov 26, 2025

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
Published on: April 8, 2019
An easy to construct sub-micron resolution imaging system
Lakhi Sharma1,2, A Roy1,2,3, S Panja1,2
1CSIR - National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi, 110012, India.
Abstract:
We report an easy to construct imaging system that can resolve particles separated by [Formula: see text] 0.68 [Formula: see text]m with minimum aberrations. Its first photon collecting lens is placed at a distance of 31.6 mm giving wide optical access. The microscope has a Numerical Aperture (NA) of 0.33, which is able to collect signal over 0.36 sr. The diffraction limited objective and magnifier recollects 77% photons into the central disc of the image with a transverse spherical aberration of 0.05 mm and magnification upto 238. The system has a depth of field of 142 [Formula: see text]m and a field of view of 56 [Formula: see text]m which images a large ensemble of atoms. The imaging system gives a diffraction limited performance over visible to near-infrared wavelengths on optimization of the working distance and the distance between the objective and magnifier.
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