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Related Experiment Video

Updated: Nov 26, 2025

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
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An easy to construct sub-micron resolution imaging system.

Lakhi Sharma1,2, A Roy1,2,3, S Panja1,2

  • 1CSIR - National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi, 110012, India.

Scientific Reports
|December 14, 2020
PubMed
Summary
This summary is machine-generated.

We developed an easy-to-build imaging system capable of resolving 0.68 micrometer particles with minimal aberrations. This advanced microscope offers wide optical access and high-quality imaging for large atom ensembles.

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Area of Science:

  • Optical microscopy
  • Particle imaging systems

Background:

  • High-resolution imaging is crucial for observing fine details in scientific research.
  • Existing systems often face limitations in aberration control and optical access.

Purpose of the Study:

  • To develop and characterize a novel, easily constructible imaging system.
  • To achieve high resolution and minimize aberrations for particle imaging.

Main Methods:

  • Construction of a microscope with a specific lens configuration and working distance.
  • Characterization of optical performance, including resolution, numerical aperture, and aberration levels.
  • Evaluation of imaging capabilities for large ensembles of atoms.

Main Results:

  • The system resolves particles separated by 0.68 micrometers with minimal aberrations.
  • Achieved a Numerical Aperture (NA) of 0.33, collecting signal over 0.36 steradians.
  • Demonstrated diffraction-limited performance with up to 238x magnification and a transverse spherical aberration of 0.05 mm.

Conclusions:

  • The developed imaging system provides high-resolution, aberration-minimized particle imaging.
  • Its design offers wide optical access and efficient photon collection.
  • The system is suitable for imaging large ensembles of atoms across visible to near-infrared wavelengths.