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Related Experiment Video

Updated: Nov 26, 2025

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
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RhizoVision Crown: An Integrated Hardware and Software Platform for Root Crown Phenotyping.

Anand Seethepalli1, Haichao Guo1, Xiuwei Liu1

  • 1Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.

Plant Phenomics (Washington, D.C.)
|December 14, 2020
PubMed
Summary
This summary is machine-generated.

The RhizoVision Crown platform offers a high-throughput solution for root crown phenotyping in crops. This open-source system enables repeatable measurements for marker-assisted breeding and understanding root systems.

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Area of Science:

  • Plant Science
  • Agricultural Technology
  • Bioinformatics

Background:

  • Root crown phenotyping is crucial for marker-assisted breeding and understanding soil resource acquisition.
  • Existing imaging protocols lack optimization for high-throughput, repeatable, and robust root crown analysis.
  • A need exists for standardized, accessible platforms for crop root system research.

Purpose of the Study:

  • To introduce and validate the RhizoVision Crown platform for high-throughput root crown phenotyping.
  • To provide an integrated, open-source solution for imaging and analyzing crop root crowns.
  • To benchmark the platform's performance and utility in phenotyping soybean and wheat.

Main Methods:

  • Developed the RhizoVision Crown platform, integrating hardware (backlight, monochrome camera) and open-source software (RhizoVision Imager and Analyzer).
  • Validated the RhizoVision Analyzer using physical phantoms (copper wire) and extensive simulated root system images (10,464 ground-truth images).
  • Applied the platform to phenotype 2,799 soybean and 1,753 wheat root crowns across multiple lines.

Main Results:

  • The platform successfully captured root crown silhouettes and extracted measurements.
  • Principal component analysis revealed correlations among features in both soybean and wheat.
  • Maximum heritability estimates were 0.74 for soybean and 0.22 for wheat, highlighting species-specific considerations.

Conclusions:

  • The RhizoVision Crown platform provides a reliable, high-throughput method for crop root crown phenotyping.
  • The open-source nature of the software promotes accessibility and standardization in plant phenotyping.
  • The platform establishes a benchmark for future open plant phenotyping systems.