Optimal method for metabolic tumour volume assessment of cervical cancers with inter-observer agreement on...

Cervical cancer metabolic tumour volume (MTV) derived from [18F]-FDG PET/CT has a role in prognostication and therapy planning. There is no standard method of outlining MTV on [18F]-FDG PET/CT. The aim of this study was to assess the optimal method to outline primary cervical tumours on [18F]-FDG PET/CT using MRI-derived tumour volumes as the reference standard.

81 consecutive cervical cancer patients with pre-treatment staging MRI and [18F]-FDG PET/CT imaging were included. MRI volumes were compared with different PET segmentation methods. Method 1 measured MTVs at different SUV_max thresholds ranging from 20 to 60% (MTV₂₀-MTV₆₀) with bladder masking and manual adjustment when required. Method 2 created an isocontour around the tumour prior to different SUV_max thresholds being applied. Method 3 used an automated gradient method. Inter-observer agreement of MTV, following manual adjustment when required, was recorded.

For method 1, the MTV₂₅ and MTV₃₀ were closest to the MRI volumes for both readers (mean percentage change from MRI volume of 2.9% and 13.4% for MTV₂₅ and - 13.1% and - 2.0% for MTV₃₀ for readers 1 and 2). 70% of lesions required manual adjustment at MTV₂₅ compared with 45% at MTV₃₀. There was excellent inter-observer agreement between MTV₃₀ to MTV₆₀ (ICC ranged from 0.898-0.976 with narrow 95% confidence intervals (CIs)) and moderate agreement at lower thresholds (ICC estimates of 0.534 and 0.617, respectively for the MTV₂₀ and MTV₂₅ with wide 95% CIs). Bladder masking was performed in 86% of cases overall. For method 2, excellent correlation was demonstrated at MTV₂₅ and MTV₃₀ (mean % change from MRI volume of -3.9% and - 8.6% for MTV₂₅ and - 16.9% and 19% for MTV₃₀ for readers 1 and 2, respectively). This method also demonstrated excellent ICC across all thresholds with no manual adjustment. Method 3 demonstrated excellent ICC of 0.96 (95% CI 0.94-0.97) but had a mean percentage difference from the MRI volume of - 19.1 and - 18.2% for readers 1 and 2, respectively. 21% required manual adjustment for both readers.

MTV₃₀ provides the optimal correlation with MRI volume taking into consideration the excellent inter-reader agreement and less requirement for manual adjustment.