Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FLIM Reveals Red Light-Induced Changes in Murine Hair Follicles.

Biosensors·2026
Same author

Investigating Metabolic Alterations in Keloid Tissues via FLIM and FLIRR Analyses.

Journal of biophotonics·2025
Same author

Rapid Fluorescence Lifetime Imaging through One-Dimensional Deep Learning Optimization.

Analytical chemistry·2025
Same author

Digital Redepleted of Stimulated Emission Depletion Microscopy for Noise Reduction and Resolution Improvement.

Analytical chemistry·2025
Same author

Fluorine-Nitrogen Codoped Carbon Dots for Visualization Imaging of Nucleic Acids via Two-Photon Fluorescence Lifetime Microscopy.

Analytical chemistry·2025
Same author

Rapid Acquisition of High-Pixel Fluorescence Lifetime Images of Living Cells via Image Reconstruction Based on Edge-Preserving Interpolation.

Biosensors·2025

Related Experiment Video

Updated: Nov 26, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.3K

Improving the image quality in STED nanoscopy using frequency spectrum modulation.

Jialin Wang1, Luwei Wang1, Jia Zhang1

  • 1Center for Biomedical Optics and Photonics (CBOP), College of Physics and Optoeletronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, China.

Journal of Biophotonics
|December 14, 2020
PubMed
Summary
This summary is machine-generated.

Frequency spectrum modulation STED (FM-STED) enhances stimulated emission depletion nanoscopy image quality. This new method improves resolution and signal-to-noise ratio while minimizing sample damage and photobleaching.

Keywords:
frequency spectrum modulationimage qualitystimulated emission depletionsuper resolution

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.3K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.1K

Related Experiment Videos

Last Updated: Nov 26, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.3K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.3K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.1K

Area of Science:

  • Microscopy
  • Biophysics
  • Materials Science

Background:

  • Stimulated emission depletion (STED) nanoscopy offers high resolution for biological and material imaging.
  • Increasing depletion laser power improves STED resolution but causes sample damage, photobleaching, and noise.
  • Current STED imaging quality is limited by these detrimental effects.

Purpose of the Study:

  • To introduce a novel image processing technique, frequency spectrum modulation STED (FM-STED).
  • To enhance the signal-to-noise ratio and resolution of STED nanoscopy images.
  • To mitigate sample photodamage and fluorophore photobleaching inherent in high-power STED imaging.

Main Methods:

  • Developed a processing method based on frequency spectrum modulation.
  • Applied FM-STED to imaging experiments using fluorescent beads.
  • Tested FM-STED performance on biological cell samples.

Main Results:

  • FM-STED significantly improved the signal-to-noise ratio in STED images.
  • Enhanced resolution was achieved using the FM-STED technique.
  • Demonstrated the effectiveness of FM-STED in preserving sample integrity.

Conclusions:

  • FM-STED is an effective method for improving STED nanoscopy image quality.
  • The technique offers a viable solution to overcome limitations of high-power STED.
  • FM-STED holds promise for advanced imaging applications in biomedicine and materials science.