Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 26, 2025

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

4.2K

Dissecting the human kidney allograft transcriptome: single-cell RNA sequencing.

Elly Varma1, Xunrong Luo2,3, Thangamani Muthukumar1,4

  • 1Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, New York.

Current Opinion in Organ Transplantation
|December 14, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Can Urinary Single-Cell Transcriptomics Reveal New Dimensions of Allograft Biology?

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Benchmarking urinary cell transcriptomes for noninvasive differentiation of BK polyomavirus-associated nephropathy from T cell-mediated rejection.

JCI insight·2026
Same author

Donor macrophage depletion permits posttransplant tolerance induction in a murine islet transplant model.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Urinary single-cell transcriptomics in kidney transplantation: Elucidation of donor-recipient cellular dynamics and fibrogenic stress.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Research Highlights.

Transplantation·2026
Same author

MRI Characteristics of Cardiotoxicity and Neurotoxicity in Patients with Breast Cancer Treated with Neoadjuvant Chemotherapy: Dynamic Changes and Interrelationships.

Academic radiology·2026

Single-cell RNA sequencing (scRNA-seq) offers unprecedented molecular insights into kidney allografts. This review details scRNA-seq methods and applications for understanding immune responses in transplanted kidneys.

Area of Science:

  • Immunology
  • Genomics
  • Transplantation Science

Background:

  • RNA sequencing (RNA-seq) provides tissue-level insights but lacks single-cell resolution.
  • Single-cell RNA sequencing (scRNA-seq) allows detailed analysis of individual cell transcriptomes.
  • Understanding scRNA-seq is crucial for interpreting kidney allograft research.

Purpose of the Study:

  • To discuss the technical aspects of scRNA-seq.
  • To summarize scRNA-seq applications in human kidney allograft research.

Main Methods:

  • scRNA-seq technology for transcriptome analysis.
  • Application in human kidney allograft studies.
  • Preclinical models of kidney transplantation.

Main Results:

More Related Videos

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

4.9K
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

16.8K

Related Experiment Videos

Last Updated: Nov 26, 2025

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

4.2K
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

4.9K
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

16.8K
  • Identified transcriptional profiles of immune cells in rejecting kidney allografts.
  • Revealed myeloid cell differentiation trajectory from monocytes to macrophages in preclinical models.
  • Highlighted the role of Axl in intragraft myeloid and T-cell differentiation.

Conclusions:

  • scRNA-seq provides high-resolution molecular data for kidney allograft research.
  • This technology elucidates cellular mechanisms of immune responses and rejection.
  • Further application of scRNA-seq will advance understanding of kidney transplantation.