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Determination of ER in ovarian cancer using monoclonal antibody technology
G B Nardelli1, V Lamaina, M Dal Pozzo
1Institute of Obstetrics and Gynaecology, University of Padua, Italy.
Clinical and Experimental Obstetrics & Gynecology
|January 1, 1987
Abstract:
The Monoclonal Antibody technology has been used in 29 cases of ovarian cancer. The immuno-enzyme-assay detected positive levels of estrogen cytoplasmatic receptor in 51.8% of the cases as well as in the nuclear (51.8%). Moreover, the ER/EIA technique screened positive levels of Total ER (ER/t) in 72.4% versus 65.5% obtained by DCC-method. 24.1% of the cases had negative ER/t levels observed by EIA. The monoclonal antibody anti-ER is a very interesting method for studying hormone-dependent tissue, because it uses an immunological binding to antigenic protein (receptor).