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Updated: Nov 26, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
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Sample pooling strategies for SARS-CoV-2 detection.

Nefeli Lagopati1, Panagiota Tsioli1, Ioanna Mourkioti1

  • 1Molecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National Kapodistrian University of Athens (NKUA), Greece.

Journal of Virological Methods
|December 14, 2020
PubMed
Summary

Pooling samples for COVID-19 testing optimizes resource use and speeds up screening. This method reduces reagent and supply needs by testing batches, with individual tests only for positive pools.

Keywords:
Array testingHierarchical algorithmN genesPooling methodsSARS-CoV-2

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Area of Science:

  • Public Health
  • Infectious Disease Diagnostics
  • Laboratory Medicine

Background:

  • The COVID-19 pandemic created an urgent need for efficient diagnostic and screening methods.
  • Accurate and rapid testing is crucial for controlling disease spread.
  • Optimized management of resources, including reagents, consumables, and personnel, is essential.

Purpose of the Study:

  • To present a pooling method for COVID-19 testing to enhance efficiency.
  • To explore hybrid methods combining "in house" protocols with commercial consumables.
  • To improve the exploitation of laboratory personnel and supplies for rapid population screening.

Main Methods:

  • A pooling strategy involving mixing individual samples into batches for initial testing.
  • Individual sample testing is performed only when a pooled sample yields a positive result.
  • Development of "in house" protocols using commercially available laboratory consumables.

Main Results:

  • Pooling samples significantly increases the number of tests that can be performed.
  • Reduces the consumption of laboratory supplies and time required for testing.
  • Enables faster screening of larger populations.

Conclusions:

  • Sample pooling is an effective strategy for optimizing diagnostic resource allocation during pandemics.
  • Hybrid "in house" methods with pooling offer a viable solution for rapid and cost-effective COVID-19 screening.
  • Efficient use of personnel and supplies through pooling facilitates timely public health responses.