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Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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Modern isolation and separation techniques for extracellular vesicles.

Thanaporn Liangsupree1, Evgen Multia1, Marja-Liisa Riekkola1

  • 1Department of Chemistry, P.O. Box 55, FI-00014 University of Helsinki, Finland.

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|December 14, 2020
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Summary

Emerging extracellular vesicle (EV) isolation methods offer efficient, user-friendly alternatives to ultracentrifugation. These advanced techniques provide high-purity EVs for therapeutic and diagnostic applications.

Keywords:
Affinity-basedCharge-basedExtracellular vesiclesIsolationSeparationSize-based

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biotechnology

Background:

  • Extracellular vesicles (EVs) are key mediators of intercellular communication.
  • EVs have significant potential in diagnostics and therapeutics.
  • Conventional isolation methods like ultracentrifugation have limitations.

Purpose of the Study:

  • To review modern extracellular vesicle (EV) isolation and separation techniques.
  • To discuss the advantages and disadvantages of emerging EV isolation methods.
  • To highlight advancements in EV isolation for research and clinical applications.

Main Methods:

  • Focus on size-, charge-, and affinity-based EV isolation techniques.
  • Exclusion of ultracentrifugation and precipitation-based methods.
  • Discussion of combined and hyphenated techniques for enhanced EV purity.

Main Results:

  • Emerging EV isolation techniques are time-effective and easy to operate.
  • These methods yield EVs with high purity and integrity.
  • An automated on-line system combining affinity trapping and FFF enables reliable EV subpopulation isolation from plasma.

Conclusions:

  • Modern EV isolation techniques offer superior performance over conventional methods.
  • Advancements facilitate the use of EVs in therapeutic and diagnostic applications.
  • Automated systems represent a significant step forward in EV research and clinical utility.