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Updated: Nov 25, 2025

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
Opportunities for Increased Nitrogen Use Efficiency in Wheat for Forage Use
Nirmal Sharma1, Raquel Schneider-Canny1,2, Konstantin Chekhovskiy1
1Grass Genomics, Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
High nitrogen use efficiency (NUE) wheat lines produced less protein but more biomass. Breeding programs can utilize these high NUE lines to improve wheat forage production.
Area of Science:
- Agronomy
- Plant Science
- Forage Production
Background:
- Wheat is a key cool-season forage crop in the southern US.
- Optimizing nitrogen fertilization is crucial for maximizing yield and quality.
- Understanding nitrogen use efficiency (NUE) and nitrogen nutrition index (NNI) is vital for sustainable agriculture.
Purpose of the Study:
- To investigate the impact of nitrogen (N) fertilization on wheat biomass yield, quality, NUE, and NNI.
- To identify wheat cultivars with superior NUE for forage applications.
- To explore the relationship between NUE, biomass, and nutritional content.
Main Methods:
- Greenhouse and hoop house experiments were conducted using a split-plot design with three replications.
- Twenty wheat cultivars/lines were subjected to four different nitrogen rates.
- Biomass yield, crude protein content, NUE, and NNI were measured and analyzed.
Main Results:
- High-NUE wheat lines generally exhibited lower crude protein content compared to low-NUE lines.
- No cultivars reached a biomass or crude protein plateau even at the highest N rates.
- The interaction between wheat line and N rate significantly affected NUE in the hoop house but not in the greenhouse.
- NNI showed a negative correlation with NUE and biomass.
- NUE strongly correlated with shoot and total biomass, and N uptake efficiency, but less so with root biomass.
Conclusions:
- Wheat lines with high NUE can be valuable resources for breeding programs aiming to enhance forage production.
- Further research is needed to optimize N management strategies for different wheat cultivars.
- The findings provide insights into the genetic potential for improving NUE in wheat for forage.
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