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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
Published on: March 20, 2016
Engineering Bacteria to Produce Pure Phage-like Particles for Gene Delivery
Matthew Tridgett1, Maria Ababi1,2, Alexander Osgerby1
1School of Life Sciences, Gibbet Hill Campus, The University of Warwick, Coventry, CV4 7AL, U.K.
Abstract:
Natural and engineered phages have been used in many applications, but their use to deliver user-defined genetic cargoes has been hampered by contamination with replicative phage, restricting use of the technology beyond the laboratory. Here we present a method to produce transducing particles without contamination. In addition, we demonstrate the use of a helper phage-free transducing particle preparation as an antimicrobial agent. This will pave the way for the development of new phage-based technologies with greater scope than lytic phage therapy.
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