Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 25, 2025

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
09:37

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Published on: August 31, 2009

25.1K

Fluorescent ligands for dopamine D2/D3 receptors.

Anni Allikalt1, Nirupam Purkayastha1, Khajidmaa Flad1

  • 1Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Str. 10, 91058, Erlangen, Germany.

Scientific Reports
|December 15, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Selectfluor-based Polonovski Rearrangement Leading to Novel Entities for Synthetic and Medicinal Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Neurotensin receptor 2 agonism attenuates adverse cardiac remodeling in preclinical models.

Science translational medicine·2026
Same author

Toward a Random Background for Ligand Optimization.

bioRxiv : the preprint server for biology·2026
Same author

Kinetic assays to characterize fluorescent ligand binding to muscarinic acetylcholine M<sub>1</sub> receptor.

Biochemical pharmacology·2026
Same author

Large Library Docking for Polypharmacology.

Journal of medicinal chemistry·2026
Same author

The Role of Intrinsically Disordered Domains in Regulating G Protein-Coupled Receptor Signaling.

Journal of the American Chemical Society·2025

Researchers developed novel fluorescent ligands targeting dopamine D2-like receptors for neurological and psychiatric disorders. These ligands show high affinity and enable visualization of receptors on living cells, aiding drug discovery.

Area of Science:

  • Medicinal Chemistry
  • Neuroscience
  • Biophysics

Background:

  • G protein-coupled receptors (GPCRs) are crucial drug targets.
  • Dopamine D2-like receptors (D2R, D3R) are implicated in Parkinson's disease and schizophrenia.
  • Fluorescent ligands are valuable tools for studying receptor dynamics and interactions.

Purpose of the Study:

  • To design and synthesize novel fluorescent ligands for dopamine D2-like receptors.
  • To evaluate the binding affinities and functional properties of these ligands.
  • To demonstrate their utility in cellular imaging and biochemical assays.

Main Methods:

  • Synthesis of fluorescent ligands based on phenylpiperazine and indanylamine scaffolds.
  • Radioligand binding assays to determine receptor affinity.

More Related Videos

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
11:49

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Published on: July 28, 2014

41.2K
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
12:48

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo

Published on: May 12, 2016

13.5K

Related Experiment Videos

Last Updated: Nov 25, 2025

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
09:37

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Published on: August 31, 2009

25.1K
Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
11:49

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Published on: July 28, 2014

41.2K
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
12:48

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo

Published on: May 12, 2016

13.5K
  • Cellular imaging using total internal reflection fluorescence microscopy (TIR-FM).
  • NanoBRET binding assays for receptor interaction studies.

    • Main Results:

    • Developed dansyl-labeled ligands with high affinity (sub-nanomolar) for D2R and D3R.
    • Xanthene and cyanine dye-labeled ligands maintained excellent binding properties and acted as agonists.
    • Cy3B-labeled ligand visualized D2R and D3R on living cells.
    • Rhodamine-labeled ligand demonstrated efficacy in NanoBRET assays for D3R.

    Conclusions:

    • Novel fluorescent ligands targeting dopamine D2-like receptors were successfully synthesized.
    • These ligands exhibit high affinity and agonist activity, suitable for D2R and D3R research.
    • The developed probes are effective tools for cellular imaging and biochemical assays, advancing GPCR research and drug discovery.